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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Kelautan Tropis
Rabia Alghazeer
Department of Chemistry, Faculty of Sciences, University of Tripoli
Earth & Planetary Sciences

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Journal : Jurnal Kelautan Tropis

 1 
Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant Subagiyo Subagiyo; Ervia Yudiati; Nuril Azhar; Rabia Alghazeer
Jurnal Kelautan Tropis Vol 23, No 3 (2020): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v23i3.9126

Abstract

Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and  V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively.  Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol  4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth. 
Chloroform Fraction from Methanol Extract of Starfish Acanthaster planci Stimulates Catfish (Clarias sp.) Macrophage Immunomodulatory Activity Janib Achmad; Ervia Yudiati; Nuril Azhar; Rabia Alghazeer
Jurnal Kelautan Tropis Vol 26, No 1 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i1.16481

Abstract

Catfish (Clarias sp.) is Indonesia's most important and popular freshwater commodity, widely cultured and intensively grown. This study is aimed to find out the effects of the chloroform fraction from a methanol extract of Acanthaster planci on non-specific immunity in Clarias sp. Thin Layer Chromatography analysis of the methanol extract's chloroform fraction yielded three fractions: FrKl3.1, FrKl3.2, and FrKl3.3. The FrKl3.3 fraction of Acanthaster planci had a significant influence on Phagocytic Activity at doses of 0.1 (54.09 ± 8.99 %), 0.3 (48.16 ± 3.34 %), 0.5 (50.39 ± 5.71 %), and 0.7 mg/kg Body Weight (46.58 ± 0.37 %). At 0.5 mg/kg Body Weight (4.03 ± 1.40 %), the Phagocytic Index of FrKl3.2 was significantly higher than the control. At 0.5 mg/kg Body Weight (38.22 ±1.43 cell 106/ml) and 0.7 mg/kg BW (40.41 ± 1.92 cell 106/ml), the number of leukocytes/white blood cells was significantly higher than the control. Nitroblue Tetrazolium and Total Plasma Protein were not significantly different from the control. Based on the results,  the FrKl3.3 was leading on phagocytic capacity and number of leukocytes 
Carrageenan Extraction of Kappaphycus alvarezii Seaweed from Nusa Lembongan Waters Using Different Alkaline Treatments Ichoun Brawici Dhewang; Ervia Yudiati; Subagiyo Subagiyo; Rabia Alghazeer
Jurnal Kelautan Tropis Vol 26, No 2 (2023): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v26i2.17389

Abstract

Kappaphycus alvarezii is a carrageenan-producing red seaweed that is widely cultivated in Nusa Lembongan waters, Bali, Indonesia. Carrageenan is generally extracted using an alkaline base,. iIn this study three different types of alkali were used to extract carrageenan originating from Nusa Lembongan Waters. This study aims to determine the quality of the extraction. The three alkalis used were KOH, NaOH, Ca(OH)2, and distilled water as a control. The 20 g dried seaweed was weighed, washed with the tap running water, and immersed in 0.15% alkaline solution (KOH, NaOH, Ca(OH)2) as well as distilled water for 24 hrs. Followed by rinsing with running water until pH was neutral. The solution was soaked and heated at 100ºC for 2 hours with a ratio of seaweed and water 1 kg in 20 L, and filtered. The extract was mixed with 1.25% KCI. The carrageenan precipitate was put in the oven at 60-80ºC until dry for 48 hours. The yield test results showed that the use of alkaline Ca(OH)2 resulted in a yield of 29.28% better than alkaline KOH (28.18%) and NaOH (27.7%). Based on the analysis of Fourier Transform Infrared Spectroscopy (FTIR), extraction using alkaline Ca(OH)2 showed functional groups characteristic of iota-carrageenan, whereas using alkaline KOH and NaOH showed functional groups characteristic of kappa-carrageenan.
Co-Authors Achmad, M. Djanib Adi Santoso Akbar Harahap Alifia Nabilla Putri Chrisna Adi Suryono Danang Ambar Prabowo Eny Heriyati Ervia Yudiati Ervia Yudiati Ervia Yudiati Ervia Yudiati Fanny Iriany Ginzel Hidayati, Jelita Rahma Ichoun Brawici Dhewang Ichoun Brawici Dhewang Jelita Rahma Hidayati Mila Safitri Rizfa Muhammad Salauddin Ramadhan Djarod Nuril Azhar Nuril Azhar Nuril Azhar Nuril Azhar Nuril Azhar Rustadi Rustadi Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Zaenal Arifin